Dharmaconea Rnai Consortium Trc Lentiviral Shrna
Fully customizable shRNA sequence vector and lentiviral manufacturing. Select by methods other than puromycin including neomycin (G418) and FACS via reporters.shERWOOD-UltramiR shRNA reagents are next generation vector-based RNAi triggers designed using the proprietary shERWOOD algorithm developed and validated in Dr. Gregory Hannons laboratory at Cold Spring Harbor Laboratory (Knott et al 2014).An optimized formulation of two plasmids expressing key structural viral packaging genes and a heterologous viral envelope gene the MISSION Lentiviral Packaging Mix is designed to be co-transfected along with MISSION TRC shRNA clones in order to create high-titer pseudotyped lentiviral
RNA Interference (RNAi) is a gene-silencing process that targets mRNA hence lowering protein expression. The key RNAi substrates regulating this process are shRNA siRNA and miRNA.Popular Lentiviral Transfer Plasmids. The lentiviral genome (i.e. the genetic material enclosed in the viral particle) is delivered to a target cell upon infection. shRNA miRNA cDNA DNA
A short hairpin RNA or small hairpin RNA (shRNAHairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
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